Other Services

Karyotyping

Detection of chromosomal  abnormalities

G-banding, (Giemsa stain), traditional karyotyping method that can detect chromosomal abnormalities with a resolution down to 3Mb.

This service includes:

  • Expansion of cells
  • Preparation and fixation
  • Sending samples for G-banding
  • Reporting the data

Array-CGH (Comparative Genomic Hybridisation with Microarray), a technology that can detect unbalanced chromosomal abnormalities with a resolution down to 100kb.

This service includes:

  • Expansion of cells
  • Preparation gDNA
  • Sending samples to a core facility of your choice
EB formation

Pluripotency assay

Embryonic Body (EB) formation and germ layer analysis with RT-PCR. This shows if an iPS cell line has the potential to differentiate into the three germ layers. This service will take approximately one month and it includes:

  • Expanding iPS cells
  • Setup EB formation
  • Harvesting EB s after two weeks
  • RNA preparation and RT-PCR with markers for all three germ layers
  • Reporting results as gel pictures
Cell Expansion (freezing-thawing)

Providing vials of established fibroblasts, iPS and NES cells

This service includes thawing, expansion and freezing of cells to deliver the desired quantity of cryo-vials.

Sample preparation for DNA/RNA

Isolating DNA/RNA from fibroblasts, iPS cells, NES cells and neurons

Isolated DNA or RNA can be used for example for bulk and single-cell sequencing, PCRs and arrays. This service includes:

  • Thawing and expansion of cells
  • Preparation of DNA and RNA
  • Delivery to customer or a chosen core facility at SciLifeLab or Karolinska Institutet
Consulting

Consulting on project planning and design, financial planning, ethical considerations and result interpretation.

Genome-edited iPS cell lines

Establishment of isogenic iPS cell lines using CRISPR-Cas9 systems

In collaboration with the Karolinska Genome Engineering (KGE) core facility at Karolinska Institutet, we establish iPS cell lines with edited genomic region of your interest.

This service takes approximately three-four months and it includes:

  • Consulting meeting together with KGE for feasibility
  • Coordination and experimental planning
  • Thawing and expansion of iPS cells
  • Preparation and conduct nucleofection
  • Recovering treated iPS cells and expansion
  • Confirming editing efficiency in bulk (ddPCR conducted by KGE facility)
  • Single-cell expansion based on editing efficiency
  • Delivering of single-cell clones for sequencing (performed by KGE facility)
  • Reporting the data and delivering edited iPS cell lines